Illuminating Phytophthora Biology with Fluorescent Protein Tags.
Identifieur interne : 000742 ( Main/Exploration ); précédent : 000741; suivant : 000743Illuminating Phytophthora Biology with Fluorescent Protein Tags.
Auteurs : Audrey M V. Ah-Fong [États-Unis] ; Meenakshi Kagda [États-Unis] ; Howard S. Judelson [États-Unis]Source :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2018.
Descripteurs français
- KwdFr :
- Expression des gènes (MeSH), Gènes rapporteurs (MeSH), Microscopie confocale (MeSH), Phytophthora (physiologie), Protéines de fusion recombinantes (MeSH), Protéines luminescentes (génétique), Protéines luminescentes (métabolisme), Technique d'immunofluorescence (MeSH), Transformation génétique (MeSH), Étiquettes de séquences exprimées (MeSH).
- MESH :
- génétique : Protéines luminescentes.
- métabolisme : Protéines luminescentes.
- physiologie : Phytophthora.
- Expression des gènes, Gènes rapporteurs, Microscopie confocale, Protéines de fusion recombinantes, Technique d'immunofluorescence, Transformation génétique, Étiquettes de séquences exprimées.
English descriptors
- KwdEn :
- Expressed Sequence Tags (MeSH), Fluorescent Antibody Technique (MeSH), Gene Expression (MeSH), Genes, Reporter (MeSH), Luminescent Proteins (genetics), Luminescent Proteins (metabolism), Microscopy, Confocal (MeSH), Phytophthora (physiology), Recombinant Fusion Proteins (MeSH), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : Luminescent Proteins.
- chemical , metabolism : Luminescent Proteins.
- physiology : Phytophthora.
- Expressed Sequence Tags, Fluorescent Antibody Technique, Gene Expression, Genes, Reporter, Microscopy, Confocal, Recombinant Fusion Proteins, Transformation, Genetic.
Abstract
Phytophthora species cause diseases that threaten agricultural, ornamental, and forest plants worldwide. Explorations of the biology of these pathogens have been aided by the availability of genome sequences, but much work remains to decipher the roles of their proteins. Insight into protein function can be obtained by visualizing them within cells, which has been facilitated by recent improvements in fluorescent protein and microscope technologies. Here, we describe strategies to permit investigators to generate strains of Phytophthora that express fluorescently tagged proteins and study their localization during growth in artificial media and during plant infection.
DOI: 10.1007/978-1-4939-8724-5_10
PubMed: 30182233
Affiliations:
Links toward previous steps (curation, corpus...)
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Ah-Fong</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Plant Pathology, University of California, Riverside, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Kagda, Meenakshi" sort="Kagda, Meenakshi" uniqKey="Kagda M" first="Meenakshi" last="Kagda">Meenakshi Kagda</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Plant Pathology, University of California, Riverside, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Judelson, Howard S" sort="Judelson, Howard S" uniqKey="Judelson H" first="Howard S" last="Judelson">Howard S. 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Judelson</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA, USA. howard.judelson@ucr.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Plant Pathology, University of California, Riverside, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author></analytic><series><title level="j">Methods in molecular biology (Clifton, N.J.)</title><idno type="eISSN">1940-6029</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Expressed Sequence Tags (MeSH)</term><term>Fluorescent Antibody Technique (MeSH)</term><term>Gene Expression (MeSH)</term><term>Genes, Reporter (MeSH)</term><term>Luminescent Proteins (genetics)</term><term>Luminescent Proteins (metabolism)</term><term>Microscopy, Confocal (MeSH)</term><term>Phytophthora (physiology)</term><term>Recombinant Fusion Proteins (MeSH)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Expression des gènes (MeSH)</term><term>Gènes rapporteurs (MeSH)</term><term>Microscopie confocale (MeSH)</term><term>Phytophthora (physiologie)</term><term>Protéines de fusion recombinantes (MeSH)</term><term>Protéines luminescentes (génétique)</term><term>Protéines luminescentes (métabolisme)</term><term>Technique d'immunofluorescence (MeSH)</term><term>Transformation génétique (MeSH)</term><term>Étiquettes de séquences exprimées (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Luminescent Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Luminescent Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines luminescentes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines luminescentes</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Expressed Sequence Tags</term><term>Fluorescent Antibody Technique</term><term>Gene Expression</term><term>Genes, Reporter</term><term>Microscopy, Confocal</term><term>Recombinant Fusion Proteins</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Expression des gènes</term><term>Gènes rapporteurs</term><term>Microscopie confocale</term><term>Protéines de fusion recombinantes</term><term>Technique d'immunofluorescence</term><term>Transformation génétique</term><term>Étiquettes de séquences exprimées</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phytophthora species cause diseases that threaten agricultural, ornamental, and forest plants worldwide. Explorations of the biology of these pathogens have been aided by the availability of genome sequences, but much work remains to decipher the roles of their proteins. Insight into protein function can be obtained by visualizing them within cells, which has been facilitated by recent improvements in fluorescent protein and microscope technologies. Here, we describe strategies to permit investigators to generate strains of Phytophthora that express fluorescently tagged proteins and study their localization during growth in artificial media and during plant infection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30182233</PMID><DateCompleted><Year>2019</Year><Month>05</Month><Day>01</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1848</Volume><PubDate><Year>2018</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Illuminating Phytophthora Biology with Fluorescent Protein Tags.</ArticleTitle><Pagination><MedlinePgn>119-129</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-4939-8724-5_10</ELocationID><Abstract><AbstractText>Phytophthora species cause diseases that threaten agricultural, ornamental, and forest plants worldwide. Explorations of the biology of these pathogens have been aided by the availability of genome sequences, but much work remains to decipher the roles of their proteins. Insight into protein function can be obtained by visualizing them within cells, which has been facilitated by recent improvements in fluorescent protein and microscope technologies. Here, we describe strategies to permit investigators to generate strains of Phytophthora that express fluorescently tagged proteins and study their localization during growth in artificial media and during plant infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ah-Fong</LastName><ForeName>Audrey M V</ForeName><Initials>AMV</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kagda</LastName><ForeName>Meenakshi</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Judelson</LastName><ForeName>Howard S</ForeName><Initials>HS</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA, USA. howard.judelson@ucr.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008164">Luminescent Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020224" MajorTopicYN="Y">Expressed Sequence Tags</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005455" MajorTopicYN="N">Fluorescent Antibody Technique</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="Y">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="Y">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008164" MajorTopicYN="N">Luminescent Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018613" MajorTopicYN="N">Microscopy, Confocal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Cell biology</Keyword><Keyword MajorTopicYN="Y">Fluorescent protein</Keyword><Keyword MajorTopicYN="Y">Oomycete</Keyword><Keyword MajorTopicYN="Y">Transformation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>9</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>9</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>5</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30182233</ArticleId><ArticleId IdType="doi">10.1007/978-1-4939-8724-5_10</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Ah Fong, Audrey M V" sort="Ah Fong, Audrey M V" uniqKey="Ah Fong A" first="Audrey M V" last="Ah-Fong">Audrey M V. Ah-Fong</name></region><name sortKey="Judelson, Howard S" sort="Judelson, Howard S" uniqKey="Judelson H" first="Howard S" last="Judelson">Howard S. Judelson</name><name sortKey="Kagda, Meenakshi" sort="Kagda, Meenakshi" uniqKey="Kagda M" first="Meenakshi" last="Kagda">Meenakshi Kagda</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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